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Secondary active transport utilizes energy from a concentration gradient to drive an active transport If the concentration gradient is steep enough, then the Gibbs energy change for moving a molecule from high concentration to low concentration will be very negative This very negative Gibbs energy change can be coupled with a transport that would otherwise have a somewhat positive (unfavorable) Gibbs energy change The total Gibbs energy change for the coupled reaction will be negative In this way, a highly favorable passive transport can be coupled with an otherwise unfavorable transport, actively driving it forward The cell is expending energy, so the transport is active But the energy comes from energy stored in the concentration gradient The concentration gradient got this energy from a primary active transport that actively pumped ions (or molecules) from one side of the membrane to the other, thereby creating a large concentration difference Since the concentration difference originated due to a primary active transport, an active transport driven by this concentration difference is termed secondary active transport Secondary active transporters, by definition, are always cotransporters, since it is specifically the passive transport of one type of molecule or ion that is driving the secondary active transport of another molecule or ion The cotransport by a secondary active transporter can be symport (in the same direction) or antiport (in the opposite direction) Many secondary active transporters are linked to the strong sodium gradient created by sodium-potassium ATPase Examples include the symport of glucose and neutral amino acids Sodium-linked symport proteins that drive the transport of glucose and neutral amino acids are commonly found in intestinal cells

} . . . private void InitializeComponent() Create the image { list within the . . . components container this.imageListToolBar = new System.Windows.Forms.ImageList(this.components); . . . // Load the // imageListToolBar image stream // for the list this.imageListToolBar.ColorDepth = System.Windows.Forms.ColorDepth.Depth8Bit; this.imageListToolBar.ImageSize = new System.Drawing.Size(16, 16); this.imageListToolBar.ImageStream = ((System.Windows.Forms.ImageListStreamer) (resources.GetObject("imageListToolBar.ImageStream"))); this.imageListToolBar.TransparentColor = System.Drawing.Color.Transparent; . . . }

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The annotated lines merit some additional discussion. This line disposes of the components container, which in turn disposes of any components contained within this object. The controls on the form are contained within the Form object itself. As a result, the resources allocated to the controls in the form are disposed by the Form.Dispose method itself. This works for components such as the MainMenu and StatusBarPanel objects as well, since the menu is assigned to the form, and status bar panels are contained within status bar controls. This line initializes an ImageList object and assigns it to the components container. This is required to ensure that the list is properly disposed of by the Form object s Dispose method. If you create your own ImageList objects manually, be sure to dispose of the object when you are finished in order to free any Windows or file system resources assigned to the list. Like the bitmap files we created in the previous chapter, a ResourcesManager object is used to retrieve the stream of image data from a .resources file. This data is retrieved as an ImageListStream object. This object is assigned to the ImageStream property and used internally by the ImageList class to manage and access the images in the collection.

On this last point for our code, note that the MyPhotos project directory in the file system contains a MainForm.resx file that defines the binary form of the image stream for our list. This is very similar to how our bitmap images were defined for our Button objects in the previous chapter. An excerpt of this file follows. In addition to the definition of the image stream, note how the positioning of objects displayed in the component tray area of Visual Studio, such as the location of our imageListToolBar object, are also stored in this file

< xml version="1.0" encoding="utf-8" > <root> . . . <data name="imageListToolBar.Location" type="System.Drawing.Point, System.Drawing, Version=1.0.3300.0, Culture=neutral, PublicKeyToken=b03f5f7f11d50a3a"> <value>255, 17</value> </data> <data name="imageListToolBar.ImageStream" mimetype="application/x-microsoft.net.object.binary.base64"> <value> AAEAAAD/////AQAAAAAAAAAMAgAAAFpTeXN0ZW0uV2luZG93cy5Gb3JtcywgVmVyc2lvbj0xLjAuMzMw MC4wLCBDdWx0dXJlPW5ldXRyYWwsIFB1YmxpY0tleVRva2VuPWI3N2E1YzU2MTkzNGUwODkFAQAAACZT eXN0ZW0uV2luZG93cy5Gb3Jtcy5JbWFnZUxpc3RTdHJlYW1lcgEAAAAERGF0YQcCAgAAAAkDAAAADwMA . . . </value> . . . </root>

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