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2 M NaCl, 6 M urea, 002 M Tris, pH 74, and eluted in 05 mL of 4 M guanidine-HCl, 002 M Tris, pH 74 The samples are dialyzed against water and lyophilized Samples are resuspended in 100 L of 01 M ammonium acetate, pH 65, and divided into half One half is digested overnight at 37 C with 2 mU/mL Keratanase II and 2 mU/mL endo -galactosidase Digested and undigested samples, normalized for cell number, are run on a 4% 20% SDS-PAGE gradient gel, transferred to PVDF membrane, and subjected to immunoblotting with Kera-C polyclonal antibody against keratocan and monoclonal antibody J36 against keratan sulfate The digested samples will not react with J36 but will show a sharp band of 55 kDa for the keratocan Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR). how to write pdf file in asp.net c# How to Easily Create a PDF Document in ASP . NET Core Web API
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PDF - Writer . NET PDF . NET component is designed to provide developers with an easy-to-use tool for Creating PDF, Editing PDF, Merge PDF, Split PDF, Fill ... Quantitative RT-PCR is performed with SYBR Green RT-PCR Reagents according to the manufacturer s instructions The reaction is carried out for 40 cycles of 15 seconds at 95 C and 1 minute at 60 C after initial incubation at 95 C for 10 minutes Reaction volume is 50 L, containing 1 SYBR Green PCR buffer, 3 mM Mg2+ , 200 M dATP, dCTP, and dGTP and 400 M dUTP, 0025 units/mL AmpliTaq Gold polymerase, 5 L cDNA, and forward and reverse primers (see Table 111) at optimized concentrations A dissociation curve for each reaction is generated on the Gene-Amp ABI Prism 7700 Sequence Detection System to con rm the absence of nonspeci c ampli cation Ampli cation of 18S rRNA is performed for each cDNA (in triplicate) for normalization of RNA content. ROW_NUMBER() OVER (ORDER BY recency) RANK() OVER (ORDER BY recency) DENSE_RANK() OVER (ORDER BY recency) how to write pdf file in asp.net c# Generate PDF File at Runtime in ASP . Net - C# Corner
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