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Stage 4 A preferred approach to calculating biochemical free energy yield using electron potential and Figure 39 Reduction potentials of many redox half-reaction pairs are shown graphically in Figure 39 The amount of energy that can be released from two half reactions can be calculated from the differences in reduction potentials of the two reactions and from the number of electrons transferred The further apart the two half reactions are, and the greater the number of electrons, the more energy is released The conversion of potential difference to free energy is given by the formula G = nF E where n is the number of electrons, F is the Faraday o constant (9648 kJ/V), and E is the difference in potentials (electron acceptor minus eleco tron donor).

Energetically favorable oxidations are those in which the ow of electrons is from (reduced) electron donors (lower right in Figure 39) up to (oxidized) electron acceptors (upper left in Figure 39) (see Section 38) Example: hydrogen gas oxidation by microorganisms using molecular oxygen and nitrate as terminal electron acceptors The couple 2H+/H2 has the potential of 041 V and the 1/2O2/H2O pair has a potential of +082 V The potential difference is 123 V, which (because two electrons are involved) is equivalent to a free energy yield ( G ) of 23734 kJ [= ( 2)(9648 kJ/V)(123 V)] By contrast, if oxygen is unavailable and anaerobic nitrate respirers (producing nitrite) are active, the potential difference between the 2H+/H2 and the NO3/NO2 reactions is 084 V, which is equivalent to a free energy yield of 16208 kJ [= ( 2)(96.

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To create a square: You can easily make your rectangle into a square by pressing Shift while you drag out your shape. Unlike some other graphics programs, you don t have to press Shift before you begin drawing; pressing Shift at any time while you re drawing causes Fireworks to increase the shorter sides to match the longer sides of the rectangle to form a square. To draw from the center: To draw your rectangle from the center instead of from the corner, press Alt (Option) when dragging out the shape. While Alt (Option) is held down, Fireworks uses the distance from your originating point to the current pointer position as the radius, rather than the diameter of the shape. As with Shift, you can press Alt (Option) at any time when drawing to change to a center origin, as shown in Figure 5-6. Spacebar Move: While not a typical keyboard modifier key, I ve included it here because of the way it impacts your workflow. In those circumstances in which you begin drawing your rectangle, only to find that it s not quite where you want it, you can hold down the spacebar, reposition your work, release the spacebar, and continue dragging out your rectangle. This can be a huge time saver.

48 kJ/V)(084 V)] Because many biochemical reactions are two-electron transfers, it is often useful to give 2 energy yields for two-electron reactions, even if more electrons are involved Thus, the SO4 2 /H2 redox pair involves eight electrons, and complete reduction of SO4 with H2 requires 4H2 (equivalent to eight electrons) From the reduction potential difference between 2H+/H2 2 and SO4 /H2S (019 V), a free energy yield of 14664 kJ is calculated, or 3666 kV per two electrons..

tMessage.text = Setting pan to + sndTrack.getPan(); } function togglePlayback(sCommand:String):Void { if(sCommand == start ) { sndTrack.start(); setButtonState( stop ); } else if(sCommand == stop ) { sndTrack.stop(); setButtonState( start ); } else if(sCommand == pause ) { nResumeSeconds = sndTrack.position / 1000; sndTrack.stop(); setButtonState( resume ); } else if(sCommand == resume ) { sndTrack.start(nResumeSeconds); setButtonState( pause ); } } function setButtonState(sCommand:String):Void { if(sCommand == stop || sCommand == start ) { tStartStop.htmlText = <a href= asfunction: togglePlayback, + sCommand + > + sCommand + </a> ; } else { tPauseResume.htmlText = <a href= asfunction: togglePlayback, + sCommand + > + sCommand + </a> ; } } function loadMP3(sType:String):Void { sndTrack.loadSound(tURL.text, sType == progressive true : false); nProgressInterval = setInterval(checkProgress, 100); setButtonState(sType == progressive stop : start ); } function checkProgress():Void { var nLoadedBytes:Number = sndTrack.getBytesLoaded(); var nTotalBytes:Number = sndTrack.getBytesTotal(); var nPercent:Number = Math.round((nLoadedBytes/ nTotalBytes) * 100); tMessage.text = nPercent + % of the sound has downloaded ; }

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