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Remove the fixative and rinse cells with HMEM several times Incubate for 30 min at 5 C in 5 M C6-bodipy-ceramide/BSA complex in a refrigerator Ceramide is a lipid that partitions to certain lipid environments in the cell and BSA serves as a carrier for the lipid Remove and save the ceramide, rinse the cells at 22 C with HMEM containing 34 mg/mL delipidated BSA several times over 1 hr, and then mount and examine the cells (The BSA removes excess ceramide and improves the specificity of labeling) If things go well, you re in for a treat! Make drawings of the Golgi apparatus from several cells Are there any indications of staining of pre-Golgi or post-Golgi structures As an alternative, label living cells and observe the pathway of dye uptake and partitioning from the plasma membrane to endosomes to the Golgi Wash a coverslip 3 with HMEM.

13421 The ID-1 Card The rst mobile network to employ a smart card for the authentication of the subscriber to the network was the analogue network Netz-C of the Deutsche Bundespost (later Deutsche Telekom) This subscriber card had evolved from a magstripe device just like a credit card, via a card having a memory chip containing the subscription details, to an IC card containing a microprocessor chip for authentication and other purposes As such smart cards were already deployed in the eld, it was not surprising that this solution was also adopted for GSM Looking at it from today s perspective, it is interesting to note how much time was devoted to certain aspects of the IC card or ID-1 SIM (ID-1 is the standardised name for cards having this format).

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TABLE 7-4. Annual water dispersal of seeds and baldcypress swamps Mean (x SE) number of water-dispersed seeds (m -2 yr -1) and total seeds (alive and dead) collected in aquatic and aerial trapsa, June 3, 1993, through May 30, 1995, in Buttonland Swamp, Illinois 1993 94 Aquatic 2 7 0 0 0 2 6 25 3 12 325 1979 0 2 10 26 8356 13742 0 0 3 11 3 13 1 4 15 33 7 17 3 10 40 170 4 22 98 153 2 10 1 3 Aerial 1 7 6 32 1 3 2 7 1 7 1 4 1 6 1212 3520 1 3 1 4 9 29 6 29 1 8 0 0 11 39 215 642 0 0 110 338 212 187 44 213 1994 95 Total

Place the coverslip in 5 M ceramide/BSA for 15 min at 37 C, then replace the solution with complete culture medium and incubate for an additional 30 min (for endosomes) or 60 min (for Golgi) at 37 C Ethidium bromide/nucleus: After rinsing in HMEM, the cells are fixed in 4 mL 05% glutaraldehyde in HMEM for 15 min Remove the fixative, dehydrate the cells in 100% ethanol for 1 min, and rinse in HMEM Add ethidium bromide to 5 g/mL (alternatively, DAPI to 05 g/mL) in HMEM, being careful not to.

It was generally assumed that most SIMs would have this format and that they might also be used as, say a payment card outside the mobile For this reason ID-1 SIMs were allowed to be embossed like a credit card and even today the slot of an ME supporting an ID-1 SIM has to be designed to cater for an embossed SIM No such SIM saw a subscriber With the change of the business model, the SIM manufacturers stopped the extensive and mostly unsuccessful testing The new card material needed to satisfy the high temperature requirements of GSM imposed on the SIM, was not really suited for embossing The tests resulted, in most cases, in pretty warped cards A much discussed and thought to be typical scenario for the SIM was its use in a car phone.

would then vote against the commit. Another reason could be that the transactional server has crashed at some point after registering its participation but before returning its vote. The coordinator will then receive an exception, time-out or other failure indication saying that the transactional server is unavailable, which it will interpret as a vote against the commit. Finally, the transactional coordinator itself may decide that it is not a good idea to commit and vote against the commit itself.

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5) Homoconjugation occurs slightly even in am-

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